| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nelson, Abigail P (12)
|
ADDACC |
36.53 |
34.26 |
-2.27 |
| 2 |
Chen, Min-syun (12)
|
ADSARY |
36.45 |
37.43 |
0.98 |
| 3 |
Hurff, Natasha B (11)
|
ADSARY |
36.37 |
37.84 |
1.47 |
| 4 |
Mohamed, Amber H (11)
|
ADSSCY |
37.23 |
37.91 |
0.68 |
| 5 |
Stark, Nikki T (11)
|
ADSARY |
45.61 |
39.89 |
-5.72 |
| 6 |
Zebrowski, Olivia G (12)
|
ADSARY |
40.72 |
40.85 |
0.13 |
| 7 |
Armstrong, Chloe V (11)
|
ADDACC |
42.25 |
42.19 |
-0.06 |
| 8 |
Wuttke, Allison M (12)
|
ADGLEN |
41.01 |
42.76 |
1.75 |
| 9 |
Taormina, Linaya E (12)
|
ADSSCY |
|
43.24 |
- |
| 10 |
Schager, Kierra G (12)
|
ADDACC |
44.80 |
44.97 |
0.17 |
| 11 |
Thompson, Madison C (12)
|
ADSARY |
46.38 |
45.28 |
-1.10 |
| 12 |
Krein, Alanna (11)
|
ADGLEN |
49.87 |
46.71 |
-3.16 |
| 13 |
Krein, Ava (11)
|
ADGLEN |
49.10 |
47.31 |
-1.79 |
| 14 |
Dupuree, Mikayla (11)
|
ADGLEN |
52.75 |
49.37 |
-3.38 |
| 15 |
Cook, McKenna R (11)
|
ADSARY |
51.22 |
50.06 |
-1.16 |
| 16 |
Boomhower, Brianna E (11)
|
ADSSCY |
49.03 |
50.27 |
1.24 |
| 17 |
Johnson, Stephanie P (11)
|
ADSSCY |
47.96 |
53.97 |
6.01 |
| 18 |
Johnson, Erin (11)
|
ADDACC |
1:00.25 |
54.62 |
-5.63 |
|
Dunn, Kaylie A (11)
|
ADDACC |
50.01 |
DQ |
- |
|
Goffioul, Clara Marie A (11)
|
ADSARY |
37.70 |
NS |
- |
|
Thrall, Lydia B (12)
|
ADSARY |
38.67 |
NS |
- |
|
Albee, Rowan (11)
|
ADDACC |
58.01 |
NS |
- |